Reporter
GG99

Part:BBa_K2145124:Design

Designed by: Katie Maruna, Laura Chen, Melody Wu   Group: iGEM16_Alverno_CA   (2016-10-18)


GFP/RFP reporter (Forward/Reverse) with random 500bp spacer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 825
    Illegal AgeI site found at 937
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This plasmid was assembled using Golden Gate Assembly.


Source

The RFP part is BBa_J04450 from the iGEM registry and the GFP part is BBa_I13522 from the iGEM registry. The backbone is a pSB1C3 backbone and the spacer is derived from a number of different backbones.

References